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KMID : 0043320080310121572
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Archives of Pharmacal Research
2008 Volume.31 No. 12 p.1572 ~ p.1577
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Antitumor and Antioxidant Activity of Protocatechualdehyde Produced from Streptomyces lincolnensis M-20
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Kim Kyoung-Ja
Kim Mi-Ae
Jung Jee-Hyung
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Abstract
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We characterized the biological functions of protocatechualdehyde (PA) isolated from the butanol extract of culture supernatant from Streptomyces lincolnensis M-20. Following butanol extraction, it was purified by silica gel and Sephadex LH-20 column chromatography. PA was analyzed by Furier Transform Infrared Spectroscopy (FT-IR), Gas chromatograph-Mass Spectrometer (GC-MS), and Nuclear Magnetic Resonance (NMR). PA had potent antioxidant activity, as measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Antitumor activity against MCF-7 human breast cancer cells was evaluated by the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide (MTT) assay. PA treatment (0~150 μM) dose-dependently blocked apoptosis, as shown by improved cell viability and inter-nucleosomal DNA fragmentation. Our findings suggest that Streptomyces lincolnensis M-20, a lincomycin producer, also produces protocatechualdehyde.
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KEYWORD
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Protocatechualdehyde, Streptomyces lincolnensis, Antioxidant activity, DPPH radical scavenging activity, Antitumor activity, MCF-7 cells
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